With the increased availability of genome sequences for bacteria, it has become routine practice to construct genomebased phylogenies. Paces7 was identified from the lignocellulolytic bacterium, pantoea ananatis sd1 as a new carbohydrate esterase. Upgma dendrogram based on fluorescent amplified fragment polymorphism analysis of selected strains of species of the genus pantoea using the selective primer combination ecocmsegc. Pantoea agglomerans, a bacterium associated with plants,is not an obligate infectious agent in humans. Pantoea ananatis is a member of the family enterobacteriaceae and is characterised by its ubiquity in nature and its frequent association with both plant and animal hosts. Maize plants inoculated with those isolates showed a pathogenic reaction. The selective efficacy of the media was tested using 89 isolates of pantoea species, 23 of p. Characterization of two pantoea strains isolated from. Pdf pantoea ananatis causes disease symptoms in a wide range of economically important. Kenyon 1 department of evolution, ecology, and organismal biology, the ohio state university. Research article open access analysis of the pantoea. Gene content of type vi secretion system 2 t6ss2 in sequenced strains of pantoea ananatis. Some species show quorum sensing ability that could drive different gene expression, hence controlling certain. Download mendeley download endnote on the structure and function of the phytoene desaturase crti from pantoea ananatis, a membraneperipheral and faddependent oxidaseisomerase.
Apr 23, 2009 pantoea ananatis, a member of the enterobacteriacea family, is a new and promising subject for biotechnological research. Plant inoculation experiments revealed that each of these strains exhibited a different. All of the 28 pantoea isolates, except bud 10 not shown, were more similar to p. Detection of pantoea ananatis in the early growing season is important for disease prediction and manage ment. There is, however, limited knowledge on the potential determinants used for host association and pathogenesis in animal systems. Pantoea ananatis serrano representatives are known to have a broad host range including both humans and plants. Whole genome sequence of pantoea ananatis r100, an. Characterization of pantoea ananatis isolated from garlic and. Nov 30, 2019 the selective efficacy of the media was tested using 89 isolates of pantoea species, 23 of p. The plant pathogen pantoea ananatis produces nacylhomoserine. Identification of the corn pathogen pantoea stewartii by mass spectrometry of wholecell extracts and its detection with novel pcr primers. Bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and dieback collected in uganda, uruguay and argentina and from maize displaying brown stalk rot symptoms in south africa were tentatively placed in the genus pantoea on the basis of phenotypic and biochemical tests. Phytophagous pentatomid insects can negatively impact agricultural productivity and the brown marmorated stink bug halyomorpha halys is an emerging invasive pest responsible for damage to many fruit crops and ornamental plants in north america.
However, it could be a cause of opportunistic human. These isolates, together with two strains lmg 2558 and lmg 2560. In a previous study, we developed a nondestructive assay that demonstrated an association between p. Biotechnology consultation note to file bnf 000156. Involvement of fenton chemistry in rice straw degradation. Lignocellulolytic bacteria have revealed to be a promising source for biofuel production, yet the underlying mechanisms are still worth exploring.
This file contains all genes found within the contiguous region representing type vi secretion system 2 pa t6ss2 in different strains of pantoea ananatis. In this study, we have evaluated the occurrence of microorganisms in 1yearstored extravirgin olive oil samples. Center rot of onion, caused by pantoea ananatis, was first reported on onion in georgia in 1997 and has continued to reduce yields and cause postharvest losses. Draft genome sequence of pantoea ananatis strain lmg 2665t. Pdf isolation and identification of pantoea ananatis from onion. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader. We characterized 36 pantoea strains, including 28 strains of diverse origins initially identified as p. Annette wensing,1,3 stefan zimmermann,2 and klaus geider3 school of engineering and science, jacobs university bremen, campus ring 1, bremen.
Pantoea ananatis erwinia ananas erwinia uredovora causes pink disease in pineapple ananas comosus, leafspot in syngonium syngonium podophyllum, purple stain in papaya carica papaya, and cereal rust. Frontiers the genomes of closely related pantoea ananatis. This bacterium can survive and multiply in a variety of ecological niches as a saprophyte, endophyte, epiphyte and pathogen. The bacterial onion pathogen pantoea ananatis, which lacks the virulence type iii and type ii secretion systems, induces cell death and necrotic symptoms on onions. Pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. Pantoea ananatis is a member of the family enterobacteriaceae and an enigmatic plant pathogen with a broad host range. These phylogenies have formed the basis for various taxonomic decisions, especially for resolving problematic relationships between taxa. The seed as a habitat for microorganisms is as yet underexplored and has quite distinct characteristics as compared to other vegetative plant tissues. Recently, several papers also described its biocontrol ability.
Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. Sep 29, 2014 download pdfp commandline program that helps you print multiple pdf files simultaneously, while allowing you to set the orientation and enter the number of copies to print. Pantoea ananatis causing grain discoloration is widely distributed in the ricegrowing fields of the republic of korea kim sang min, national institute of crop science, republic of korea ah107 title. To identify genes involved in lignocellulose degradation, cds were analyzed using dbcan carbohydrateactive. In this study, we used the model host dictyostelium discoideum to show that. A semiselective medium to isolate and identify bacteria. Pantoea agglomerans annals of agricultural and environmental. Crystal structure of a sugar nformyltransferase from the plant pathogen pantoea ananatis. Identification of the corn pathogen pantoea stewartii by mass. Over recent years, impressive progress in its application to lglutamate production has been achieved.
Results that are typical of the pantoea isolates are shown for nine isolates which are compared with the type strains of p. Pantoea agglomerans is a gramnegative bacterium that belongs to the family erwiniaceae it was formerly called enterobacter agglomerans, or erwinia herbicola and is an ubiquitous bacterium commonly isolated from plant surfaces, seeds, fruit, and animal or human feces and can be found throughout a honeybees environment. Azte00000000 comprises a total of 4927,500 bp containing 4332 protein coding sequences cds detailed results were shown in additional file 1. In this study, whole genome sequence of this strain was determined by smrt. Phylogeny and identification of pantoea species and typing of. This article is from frontiers in bioengineering and biotechnology, volume 2. Genomic and secretomic insight into lignocellulolytic.
Pantoea agglomerans and other pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of pantoea strains and their possible association with hosts and disease remain poorly known, and identification of pantoea species is difficult. The rice endophytic bacterium pantoea ananatis sd1 with strong lignocellulose degradation capacity has been reported in our previous study. The olive oil is an unfavorable substrate for microbial survival and growth. May 10, 2016 pantoea ananatis is a group of bacteria, which was first reported as plant pathogen. Frontiers pantoea ananatis genetic diversity analysis. Download fulltext pdf download fulltext pdf download fulltext pdf. Isolation of pantoea ananatis from sugarcane and characterization. Reports of both human and plant pathogenic strains are well documented 1. However, a comprehensive analysis of its corresponding degradative system has not yet been conducted. Characterization of pantoea ananatis isolated from garlic. Pantoea ananatis causes disease symptoms in a wide range of.
Detection of pantoea ananatis, causal agent of leaf spot disease of. May 12, 2015 the seed as a habitat for microorganisms is as yet underexplored and has quite distinct characteristics as compared to other vegetative plant tissues. Research article open access analysis of the pantoea ananatis. Pantoea ananatis causes disease symptoms in a wide range of economically important agricultural crops and forest tree species worldwide. Pantoea ananatis is a gramnegative, motile, facultative anaerobe belonging to the gamma proteobacteria. Species of the genus pantoea, a member of the enterobacteriaceae, were identified on thorns of common hawthorn and cotton palm spines table 4. Habitat visualization and genomic analysis of candidatus pantoea carbekii, the primary symbiont of the brown marmorated stink bug laura j.
A novel glycolipid biosurfactant confers grazing resistance. Pantoea ananatis lmg 2665t synthesizes and utilizes acyl homoserine lactones ahls for signalling. Pairwise comparisons of lmg 2665t in the absence of ahls optical density od600. Pantoea ananatis defeats allium chemical defenses with a. The combination of both methods allows a rapid and simple identification of the corn pathogen. Pantoea ananatis is a group of bacteria, which was first reported as plant pathogen. Abstractthe biotechnologically relevant bacterium corynebacterium glutamicum. Nevertheless, genetic and biotechnological studies of pantoea ananatis have been impeded because of the absence of. The cotton fleahopper pseudatomoscelis seriatus, reuter is a significant pest that causes cotton bud damage that may result in significant yield losses. They were isolated from rice seeds and leaves in paddy fields or from seed stored in benin and togo. The synergistic effect of concatenation in phylogenomics. Blastn analysis of a 1500 bp 16s rdna gene sequence genbank accession no. Draft genome sequence of pantoea ananatis strain mhsd5. Identification of pantoea ananatis gene encoding membrane.
Transcriptome profiling reveals the eanir quorum sensing. Eight yellowpigmented, gramnegative, rodshaped, oxidasenegative, motile, facultatively anaerobic bacteria were isolated from onion seed in south africa and from an onion plant exhibiting centre rot symptoms in the usa. It is regarded as an emerging pathogen based on the increasing number of reports of diseases occurring on previously unrecorded hosts in different parts of the world. In this study, we investigated three closely related p. Despite the popularity of concatenating shared genes to obtain wellsupported. Analysis of the pantoea ananatis pangenome reveals. With the exception of perhaps only pantoea agglomerans, no other plant pathogen. Phylogeny and identification of pantoea species and typing. Pantoea deleyi and pantoea eucalypti produce bacterial blight and dieback of eucalyptus leaves.
Tolerance of castanea sativa cultivars to dryocosmus kuriphilus tiziana panzavolta and matteo, bracalini university of florence, italy ah108. This is the first report of a disease of mexican maize caused by p. Transmission of pantoea ananatis, causal agent of center. Pantoea ananatis, a member of the enterobacteriacea family, is a new and promising subject for biotechnological research. First report of pantoea ananatis in japonia rice varieties in turkey. The isolates were assigned to the genus pantoea on the basis of phenotypic and biochemical tests. We have identified a plasmidborne cluster of sulfurredox.
Download pdfp commandline program that helps you print multiple pdf files simultaneously, while allowing you to set the orientation and enter the number of copies to print. May 27, 2014 pantoea ananatis is found in a wide range of natural environments, including water, soil, as part of the epi and endophytic flora of various plant hosts, and in the insect gut. Pantoea bacteria are yellow pigmented, ferment lactose, are motile, and form mucoid colonies. In this study, rnasequencing rnaseq was used to identify the eanir regulon in lmg 2665t. May 01, 2009 summary pantoea ananatis causes disease symptoms in a wide range of economically important agricultural crops and forest tree species worldwide. We present here the draft genome sequence and annotation of p. Transmission and importance of pantoea ananatis during. In this study, we used the model host dictyostelium discoideum to show that isolates of pantoea ananatis exhibit. Save map as png save map as svg save list as excel file save list as csv file. Pairwise comparisons of lmg 2665t in the absence of ahls. Plant inoculation experiments revealed that each of these.
Comparative genomics of type vi secretion systems in strains. It is regarded as an emerging pathogen based on the. A total of 36 strains belonging to the genus pantoea were used. Abstractwe report the draft genome sequence of pantoea ananatis lmg 2665t, the bacterial causal agent of. Its unconventional nature lies in the fact that, unlike the majority of plant.
Pantoea is a versatile genus of bacteria with both plant and animalpathogenic strains, some of which have been suggested to cause human infections. A number of gramnegative bacteria have a quorumsensing system and produce nacyllhomoserine lactone ahl that they use them as a quorumsensing signal molecule. Comparative genomics of type vi secretion systems in. These enzymes catalyze the conversion of geranylgeranyl. Pantoea species are ubiquitous in nature and occasionally associated with infections. To our knowledge this is the first report of the presence of the p. A semiselective medium to isolate and identify bacteria of. Only few microorganisms use olive oil fatty acids as carbon and energy sources, and survive in the presence of olive oil antimicrobial components. Exploring microorganisms especially bacteria associated with the degradation of lignocellulosic biomass shows great potentials in biofuels production. The complete set of genes regulated by the eanir quorum sensing qs system in this strain is still not fully known. Pantoea is a genus of gramnegative bacteria of the family erwiniaceae, recently separated from the genus enterobacter. Pantoea ananatis strain mhsd5 is a bacterial endophyte isolated from the surface sterilized leaves of pellaea calomelanos, which is a medicinal plant obtained in limpopo province of south africa.
This article is from genome announcements, volume 2. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer. We present complete genome of pantoea ananatis r100, which is an. It has been found in a wide array of environments including rivers, soil samples, refrigerated beef and aviation fuel tanks 1, 2. Pantoea ananatis promotes growth of arabidopsis plants 15305 genetics and molecular research 14 4.
Pantoea ananatis british society for plant pathology. Lycopene is the precursor to provitamin a carotenoids, including. Identification and characterization of a novel bacterial. If you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs. Some strains have proven effective as biological control agents and plantgrowth promoters, while other strains have been implicated in diseases of a broad range of plant hosts and humans. Habitat visualization and genomic analysis of candidatus. Genomic and secretomic insight into lignocellulolytic system. In this study, a bacterial strain previously isolated from a fleahopper was tested for cotton infectivity using. Pronunciation of pantoea with 2 audio pronunciations, 1 translation and more for pantoea.